Category: Western Blot

The Acidities of Nucleophilic Monofluoromethylation Reagents: An Anomalous α-Fluorine Effect

Fluorine is essentially the most electronegative ingredient within the Periodic Table. Fluorine incorporation into natural molecules is predicted to decrease the p Okay a of neighboring performance by way of its sturdy electron-withdrawing impact, and this technique has been broadly exploited in various disciplines. Herein, we report a hanging anomalous a -fluorine substitution impact on

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α-Amino Acids and Peptides as Bifunctional Reagents: Carbocarboxylation of Activated Alkenes via Recycling CO 2

Carboxylic acids, together with amino acids (AAs), have been broadly used as reagents for decarboxylative couplings. In distinction to earlier decarboxylative couplings that launch CO2 as a waste byproduct, herein we report a novel technique with simultaneous utilization of each the alkyl and carboxyl parts from carboxylic acids. Under this distinctive technique, carboxylic acids act as

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Computational and Experimental Study of Turbo-Organomagnesium Amide Reagents: Cubane Aggregates as Reactive Intermediates in Pummerer Coupling

The dynamic equilibria of organomagnesium reagents are recognized to be very complicated, and the relative reactivity of their parts is poorly understood. Herein, a mix of DFT calculations and kinetic experiments is employed to analyze the detailed response mechanism of the Pummerer coupling between sulfoxides and turbo-organomagnesium amides. Among the varied aggregates studied, unprecedented heterometallic

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Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues

We studied the suitability of commercially accessible monoclonal antibodies (mAbs) for the immunohistochemical (IHC) detection of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV2) in normal archival specimens. Antibodies had been screened on HEK293 cells transfected with viral nucleoprotein, S1 subunit and S2 subunit of spike protein and on untransfected cells, in addition to a panel

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