The Acidities of Nucleophilic Monofluoromethylation Reagents: An Anomalous α-Fluorine Effect

The Acidities of Nucleophilic Monofluoromethylation Reagents: An Anomalous α-Fluorine Effect

The Acidities of Nucleophilic Monofluoromethylation Reagents: An Anomalous α-Fluorine Effect

Fluorine is essentially the most electronegative ingredient within the Periodic Table. Fluorine incorporation into natural molecules is predicted to decrease the p Okay a of neighboring performance by way of its sturdy electron-withdrawing impact, and this technique has been broadly exploited in various disciplines.
Herein, we report a hanging anomalous a -fluorine substitution impact on the a -C sp3 -H acidity. We have experimentally measured the p Okay a values of a collection of in style nucleophilic monofluoromethylating reagents a -fluoro(phenylsulfonyl)methane derivatives in addition to their C-H analogs by Bordwell’s overlapping indicator methodology in dimethyl sulfoxide answer.
Contrary to expectations, we discovered {that a} -fluorine substituent doesn’t usually improve however reasonably weaken the a -C sp3 -H acidity of most (phenylsulfonyl)methane derivatives. DFT computations reproduce and supply perception into the anomalous a -fluorine impact. An affordable correlation was recognized between the C-H p Okay a of (phenylsulfonyl)methane derivatives and Mayr’s nucleophilicity parameter ( N ) of the corresponding carbanions.

Optimization of Cysteine Residue Alkylation Using an On-line LC-MS Strategy: Benefits of Using a Cocktail of Haloacetamide Reagents

Several frequent reagents for the alkylation of cysteine residues of mannequin intact proteins have been evaluated for response velocity, yield of alkylated product and diploma of over-alkylation utilizing an internet LC-MS platform. The effectivity of the alkylation response is discovered to be depending on the (1) reagent, (2) peptide/protein, (3) reagent focus and (4) response time.
At excessive reagent concentrations, iodoacetic acid was discovered to supply vital ranges of over-alkylation merchandise whereby methionine residues turn into modified. For optimum efficiency of the alkylation response, we discovered the use of a cocktail of chloroacetamide, bromoacetamide and iodoacetamide labored greatest.
The alkylating effectivity of every haloacetamide is a stability between the traits of the halogen leaving group and the steric hindrance of the alkylation website on the peptide or protein. A key facet of utilizing a cocktail of haloacetamides is that all of them produce the identical modification (+57.0209 Dalton) to the cysteine residues of the protein whereas the alkylation effectivity of every website might differ for every of the three reagents. Over-alkylation results look like decrease with the cocktail on account of a decrease focus of every reagent. The haloacetamide cocktail may very well be helpful when contemplating complicated mixtures of proteins.

Mouse Preclinical Cancer Immunotherapy Modeling Involving Anti-PD-1 Therapies Reveals the Need to Use Mouse Reagents to Mirror Clinical Paradigms

Immune checkpoint inhibition (ICI) has emerged as one of essentially the most highly effective instruments to reverse most cancers induced immune suppression. Monoclonal antibodies (mAbs) focusing on programmed cell dying 1/programmed cell dying ligand 1(PD-1/PD-L1) are FDA-approved and their medical use is quickly increasing. As against the medical paradigm, which may end up in vital responses and toxicities, it has been tough to breed these results preclinically utilizing mouse fashions.
In giant half, this is because of fashions, which make use of quickly rising ex vivo cultured transplantable tumor cell traces engrafted into younger naïve inbred laboratory mice. However, one other difficulty issues the use and repeated software of xenogeneic reagents in mice (i.e., rat or hamster mAbs directed towards mouse antigens at variance with medical use of human or humanized mAbs).
The Acidities of Nucleophilic Monofluoromethylation Reagents: An Anomalous α-Fluorine Effect
Building on our earlier research demonstrating that repeated administration of generally used xenogeneic anti-PD-1 mAbs derived from each rat and hamster can induce deadly hypersensitivity in some tumor-bearing mice, we sought to check these end result with the consequences of a mouse anti-mouse PD-1 mAb. Application of a murine anti-mouse PD-1 (clone: MuDX400) didn’t end in deadly anaphylaxis within the 4T1 tumor mannequin.
It additionally displayed superior antitumor results on this and different tumor fashions, because it didn’t induce neutralizing antibody responses towards the anti-PD-1 mAb, corresponding to have been noticed when utilizing xenogeneic anti-PD1 mAbs. These outcomes exhibit that extra correct preclinical modeling necessitates the use of mouse reagents mirroring the medical situation to determine long-term results or toxicities, whereas avoiding xenogeneic responses, which don’t happen clinically. Furthermore, these research recommend a direct mechanism, whereby preclinical murine research have typically did not recapitulate the medical efficacy and toxicity of single agent checkpoint inhibition.

Experimental and Computational Evaluation of Chloranilic Acid as an Universal Chromogenic Reagent for the Development of a Novel 96-Microwell Spectrophotometric Assay for Tyrosine Kinase Inhibitors

The tyrosine kinase inhibitors (TKIs) are chemotherapeutic medicine used for the focused remedy of varied sorts of most cancers. This work discusses the experimental and computational analysis of chloranilic acid (CLA) as a common chromogenic reagent for growing a novel 96-microwell spectrophotometric assay (MW-SPA) for TKIs. The response resulted in an instantaneous formation of intensely purple coloured merchandise with TKIs. Spectrophotometric outcomes confirmed that the reactions proceeded by way of the formation of charge-transfer complexes (CTCs).
The bodily parameters have been decided for the CTCs of all TKIs. Computational calculations and molecular modelling for the CTCs have been carried out, and the location(s) of interplay on every TKI molecule have been decided. Under the optimized circumstances, Beer’s legislation correlating the absorbances of the CTCs with the concentrations of TKIs have been obeyed within the vary of 10-500 µg/properly with good correlation coefficients (0.9993-0.9998).

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OOSA10411-5ML - SERUM:Azide Free Serum & Cells

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Bovine Serum Albumin Fatty Acid Free Reagent Grade

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Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

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Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade

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Free Chlorine Reagents 0-5mg/L - EACH

WAT1028 EACH
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VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 769.2

EZ-DNA Reagents

BS8202 100preps
EUR 105.67

EZ-DNA Reagents

SK8201 100preps
EUR 112.2

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

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Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

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EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

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T-Pro Total Exosome Isolation reagent (from serum)

JO66-V002M 25ml/BT
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T-Pro Total Exosome Isolation reagent (from serum)

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Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!

P5W13 10 rxn
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Ecl Detection Reagents

RPN3004 EACH
EUR 552.9

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

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Human IgG (serum origin, purified >97%, low endotoxin, azide free)

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EUR 196.8

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade

OPEF01351-100G 100g
EUR 1302

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)

WA-013 each
EUR 135

CRP Free Serum

GWB-FD777C 50 ml Ask for price

Ecl Detection Reagents - EACH

RPN2105 EACH
EUR 815.4

Steroid Free Serum

90R-1007 50 mL
EUR 363
Description: Steroid Free Human Serum

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 343.2

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

REAGENTS HIGH RANGE AMMONIA - PK25

HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH

HI9373301 EACH
EUR 130.95

Myeloperoxidase Free Serum

GWB-B28A30 50 ml Ask for price

Troponin I Free Serum

GWB-38F44D 50 ml Ask for price

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Apo AI/B Free Serum

GWB-9D7839 100 ml Ask for price

PeliKine tool set, additional reagents

M1980 1 unit
EUR 169.8

Alkphos Direct Labelling Reagents - EACH

RPN3680 EACH
EUR 915.3

Human Serum (CRP free)

90R-100 100 ml
EUR 1326
Description: C-reactive protein free normal human serum

Serum Free Medium For Mouse Neural Stem Cells

MUXNF-90011 100mL
EUR 289

Human Serum (FABP free)

90R-109 100 ml
EUR 995
Description: FABP free normal human serum

Human Serum (Myoglobin Free)

90R-110 100 ml
EUR 995
Description: Myoglobin free normal human serum

HEK 293 Media, Serum Free

TBS8084-1L 1L
EUR 98

OOMA00062-50ML - CRP Free Serum

OOMA00062-50ML 50ML
EUR 1379

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 450ml
EUR 225.6

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-100 950ml
EUR 130

RAT SERUM WITH 0.09% AZIDE Serum Products

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Liposomal and Non-Liposomal Transfection Reagents

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abx298032-20g 20 µg
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Human Serum (Troponin I Free)

90R-106 100 ml
EUR 1417.2
Description: Troponion I free normal human serum

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Serum Free Cryopreservation Medium

TM026 25 ml
EUR 50

Serum Free Medium (Type II) For Mouse Embryonic Stem Cells

MUXES-90061 200mL
EUR 839

Serum Free Medium (Type I) For Mouse Embryonic Stem Cells

MUXES-90062 200mL
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Bovine Serum Albumin, RNase Free

40200064-1 1 mL
EUR 33.37

Serum Albumin, Lipid Free Protein

20-abx260049
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GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products

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Endothelial Growth Medium (EGM) Serum Free + Xeno Free

cAP-02-SF-XF 500ml
EUR 133.65

OOSA10412-5ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10412-5ML 5ml
EUR 69

Albumin, Bovine Serum, Globulin Free

01281-26 100G
EUR 265.3

Albumin, Bovine Serum, Globulin Free

01281-84 50G
EUR 153.3

Albumin, Bovine Serum, Globulin Free

01281-97 10G
EUR 43.4

Bovine Serum Albumin, protease free

GK4012-1 1
EUR 2065.2

Bovine Serum Albumin, protease free

GK4012-1KG 1 kg
EUR 1165.2

Bovine Serum Albumin, protease free

GK4012-500 500
EUR 1181.9

Bovine Serum Albumin, protease free

GK4012-500G 500 g
EUR 638.4

OOSA10414-10ML - SERUM WITH 0.09% AZIDE Serum & Cells

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OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

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500mL INSECTAGRO SF9 serum free - PK6

13-410-CV PK6
EUR 249.75

FBS - Exosome Free Fetal Bovine Serum

FBS002-X each
EUR 120.6

FBS - Exosome Free Fetal Bovine Serum

FBS002-X-A each
EUR 601.2

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EUR 1799

Serum-free Cell Freezing Medium (Ready-to-use)

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EUR 80

Bovine Serum Albumin, fatty acid free

GX5685-1 1
EUR 1162.8

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EUR 1442.4

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EUR 624.9

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Freezing Medium (Serum-free & animal origin-free)

PB180438-100mL 100 mL
EUR 118
Description: Supplements & Reagents

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EUR 65
Description: Supplements & Reagents

Freezing Medium (Serum-free & animal origin-free)

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EUR 118
Description: Others

Normal Bovine Serum (Protease/IgG free)

88R-1012 10 ml
EUR 166
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Albumin, Bovine Serum, Protease Free, pH5.2

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EUR 53.9

Albumin, Bovine Serum, Protease Free, pH5.2

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EUR 178.5

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EUR 594

MOUSE SERUM Serum Products

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OPMA04391-50ML - Troponin I Free Serum Protein

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EUR 1299

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Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-26 10G
EUR 63.7

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

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EUR 137.9

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

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EUR 184.1

Bovine Serum Albumin Protease free pH 7.0

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Mouse lambda light chain (free and bound) goat polyclonal antibody, Serum

AP31536SU-N 1 ml Ask for price

Fetal Bovine Serum, Premium Tetracyclin Free

F0500-050 500ml
EUR 1388.4

Serum Free Medium For Rat Neural Stem Cells

RAXNF-90011 100mL
EUR 289

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 5nm

22716-100 100ml
EUR 932
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 10nm

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EUR 919
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 15nm

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EUR 920
Description: 7732-18-5

ImmunoGold labeling reagents for (SEM) - Unconjugated gold colloid (GC) 20nm

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EUR 932

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EUR 56.82

Fetal Bovine Serum (EU Origin). Tetracycline free - 500ml

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EUR 220

Corning Lymphocyte Serum Free Medium KBM551 - 1L

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EUR 226.8

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EUR 299.7

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EUR 421.2

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RPN2209 EACH
EUR 328.05

Mouse Serum - 100ml

MO-340/100 100ml
EUR 183.32

Mouse Serum - 500ml

MO-340/500 500ml
EUR 733.32

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EUR 250

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PM-T1726/1000 kg
EUR 549.29

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EUR 56.82

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EUR 220

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PM-T1726/100 100g
EUR 111.21

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PM-T1726/500 500g
EUR 339.79

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22070023-1 5 g
EUR 22.36

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-2 10 g
EUR 27.79

Bovine Serum Albumin, Fraction V - Fatty Acid Free

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EUR 51.82

Bovine Serum Albumin, Fraction V - Fatty Acid Free

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EUR 78.47

Bovine Serum Albumin, Fraction V - Fatty Acid Free

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EUR 116.35

Bovine Serum Albumin, Fraction V - Fatty Acid Free

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EUR 206.54

Bovine Serum Albumin, Fraction V - Fatty Acid Free

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EUR 358.96

Bovine Serum Albumin, Fraction V - Fatty Acid Free

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EUR 583.54

Goat Serum (10%) in PBST with Azide

30611260-1 100 ml
EUR 33.41

Goat Serum (10%) in PBST with Azide

30611260-2 250 ml
EUR 59.36

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30611278-1 100 ml
EUR 31.76

Horse Serum (10%) in PBST with Azide

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EUR 59.79

Mouse Serum

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EUR 155407.2

Mouse Serum

20-abx098261
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Mouse Serum

abx098261-5L 5 L
EUR 10599.6

Mouse Serum

abx098262-1ml 1 ml
EUR 2064

Mouse serum

IHR-8141 10 ml
EUR 47.75

Mouse Serum

M2021-050 500ml
EUR 970

Mouse Serum

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Mouse Serum

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Fetal Bovine Serum (EU Origin). Tetracycline free - 50ml

FB-1280T/50 50ml
EUR 45.49

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PM-T1727/1000 kg
EUR 1136.69

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-5 each
EUR 144

Serum Free Medium For Human Fetal Neural Stem Cells

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EUR 289

Bovine Serum Albumin Fatty Acids Free Lyophilised - 100g

PM-T1727/100 100g
EUR 155.21

Bovine Serum Albumin Fatty Acids Free Lyophilised - 500g

PM-T1727/500 500g
EUR 574.42

Serum-Free Medium

cAP-62 250ml
EUR 133.65

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-1 100 ml
EUR 31.76

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-2 250 ml
EUR 54.54

SuperKine™ Serum/Protein-Free Cell Freezing Medium

BMU108-EN-100mL 100 mL
EUR 109
Description: SuperKine™ Serum/Protein-Free Cell Freezing Medium is a kind of non programmed, ready-to-use cell freezing medium with clear formula, serum-free and protein-free, which is applicable to most mammalian cells. It can eliminate the potential pathogen contamination or immune reaction risk of serum or protein, so it is safer to use, no need for programmed cooling, and can keep the recovery survival rate of various cells above 95%.

HSA Human Serum Albumin Recombinant Protein, Lipid Free

PROTP02768-3 Regular: 50mg
EUR 565.2
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.

SuperKine™ Serum/Protein-Free Cell Freezing Medium

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SuperKine™ Serum/Protein-Free Cell Freezing Medium

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Fetal Bovine Serum (South America). Tetracycline free - 50ml

FB-1001T/50 50ml
EUR 45.49

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 724

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-100 each
EUR 1358.4

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-25 each
EUR 535.2

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-5 each
EUR 210

Myoglobin-Free Serum

GWB-CA7EA4 50 ml Ask for price

Goat Serum (10%) in TBST Buffer with Azide

30611263-1 100 mL
EUR 33.41

Goat Serum (10%) in TBST Buffer with Azide

30611263-2 250 ml
EUR 57.19

Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents-

E-BC-K205-96T 96T
EUR 150
Description: Quantitative

Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents-

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Description: Quantitative

Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents-

E-BC-K206-S-100Assays 100 Assays
EUR 240
Description: Quantitative

Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents-

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Description: Quantitative

High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents-

E-BC-K221-96T 96T
EUR 150
Description: Quantitative

High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents-

E-BC-K221-each each Ask for price
Description: Quantitative

High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents-

E-BC-K222-S-100Assays 100 Assays
EUR 240
Description: Quantitative

High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents-

E-BC-K222-S-each each Ask for price
Description: Quantitative

Purified rat serum albumin protein (RSA, >99%, globulin free)

ALBR13-N-10 10 mg
EUR 343.2

30(w/v)%-Albumin, Bovine Serum, Solution, Fatty Acid Free

19361-84 20ML
EUR 105

Mouse Serum Proteins goat polyclonal antibody, Serum

AP31483SU-N 1 ml Ask for price

Xpro IP Cell Lysis Reagent,SDS Free

X3100-010 100ml
EUR 208.8

Xpro IP Cell Lysis Reagent,SDS Free

X3100-050 500ml
EUR 380.4

Mouse Serum Proteins rabbit polyclonal antibody, Serum

AP31472SU-N 1 ml Ask for price
The proposed MW-SPA totally validated and efficiently utilized for the dedication of all TKIs of their bulk types and pharmaceutical formulations (tablets). The proposed MW-SPA is the primary assay that may analyze all of the TKIs on a single assay system with out modifications within the detection wavelength. The benefits of the proposed MW-SPA are easy, financial and, extra importantly, have excessive throughput.