Monoclonal Antibodies for Flow


How mouse monoclonal antibody is different?

Mouse  monoclonal antibodies have great binding affinity and also these antibodies can be easily purified as compare to other antibodies. If these antibodies will be used according to the protocol then these can provide extraordinary results that could be reproducible with guaranteed success as well. Monoclonal anti bodies are quite advantageous as compare to other polyclonal antibodies because as we know that hybridoma takes a bit longer to other antibodies and after this when the line is completely ready it starts reproducing these antibodies endlessly whereas monocona antibodies are quite specific to a specific epitope and they can be use for any for certain applications wherever specific targeting is essential.

What are the specific requirements for storing such type of antibodies?

Specifically, all the antibodies requires an ambient temperature to be transported after the lyophilization process and also the storage requirement for each and every antibody is also very specific because for shorter storage period they have to be stored at 4ºC and for longer period of time they have to be stored at -20ºC. In liquid form antibodies can be transferred from one place to another at 4ºC and for longer time period they have to be stored at -20ºC by adding 25-50% solution of glycerol and ethylene glycol.

What kind of mouse monoclonal antibodies is most useful?


Brdu is an antibody that specifically reacts with only single stranded DNA it is most likely attached with the protein carrier. Detection of nucleated cells is quite easier with these antibodies as they can be incorporated into the DNA during an S phase of cell cycle. It was reported that these antibodies can be react with EdU that is 5-Ethynyl-2-deoxyuridine and also it can favorably react with CIdU that is 5-cholor-2-deoxyuridine. Moreover, these antibodies can also react with IdU.

DNA needs to be unraveled first because this antibody only recognizes single stranded DNA and this can be done by using DNase. Acid denaturation can be done by using 2M HCL depending on the assay or even heat denaturation is also effective. 

  • These antibodies can be shipped at 4ºC and after delivery they can be stored at -20–80ºC by avoiding freezing/thawing cycle with a pH of 7.4 as well.

Anti-PD1 antibody

This type of antibody basically recognizes human PD1 that is a checkpoint protein activated by certain immune cells such as B and T cells involved in some cellular responses. Host specie of this type of antibody is specifically mouse.

Mouse Monoclonal Antibody 1A4  (Alpha Smooth Muscle Actin):

This specific antibody reacts with certain smooth muscle cells, testis, ovary and certain parenchyma tissues of intestine.