GlogiStain test
This kit is really helpful in studying the morphology of both the normal and abnormal neurons by using Glogi-Cox impregnation. By using this technique subtle morphological alterations in neuronal dendrites and dendritic spines have been identified in the brains of certain animals treated with such drugs and also in some post-mortem brains of certain patients having neurological diseases. Moreover, the unreliability and the time consuming process of this technique is a major obstacle to the widespread application.
Rapid Golgistain kit is based on the principle of the methods that has dramatically simplified the Golgi Cox technique and proved itself to be extremely reliable and effective.
This kit contains 5 types of solutions
Solution A, B, C , D and E with a concentration of 250ml. It also contains glass specimen retriever and a natural hair paintbrush with a dropping bottle.
All these solutions should be enough dissolved and stirred. Another point that should be considered is that heating is required while making solution D. Also, stock solution should be kept in the dark for a few months and mix 5 volume parts of solutions A , B and C and 10 volume of solution D by stirring them. Moreover, after mixing all these solutions it should be kept in the dark for 24 hours and after this reddish precipitates will be formed.
Kit can be used to process large and even small quantities of brain samples or even sections all at once. Moreover, it should be kept in mind that only fresh and shortly fixed brain tissues should be used and previously formalin fixed or even fresh frozen brains is also not recommended because the results will not be satisfactory. Another important point that it should be kept in mind is that already cut or free floating sections should be mounted on microscope slides.
As we know this kit is only for in vitro research use only and not for the drug or even for the diagnostic use as well.
Also, the reagents present in this kit are more toxic and harmful so when there is any contact developed between the skin or even by inhalation it can be a lot more fatal. So, don’t take it through mouth pipette. If in case there is any contact wash it immediately by using plenty of water and seek medical assistance as well.
Use a chemical hood while performing the experiments and also wear protective gloves, clothing and eye protection while performing.
Solution A and B are toxic or fatal enough because of the presence of potassium dichromate, potassium chromate if they come into contact with skin. Also, don’t breathe vapour or fumes from all these solutions.
Moreover, do not pour the waste of solutions A and B into the sink. Collection of waste should be in the waste container and furthermore contact thw local safety office to dispose of the material safely.
Staining solution should be prepared in a way that it consist of one part of solution D and one part of solution E and the remaining two parts of double distilled water. So, in order to make it take 10ml of solution E and D and 20ml distilled water. One more thing that should be kept in mind is the staining solution should be prepared only before it’s usage for upto 100 sections only keeping in mind the total size of the section of brain. Moreover, keep the staining jar covered in order to prevent it from evaporation.
Counterstaining is a favourable technique it not only allows the determination of the precise location of the respective neuron but also it is effective enough to visualise the sections of anatomic landmarks that are not evident or visible without doing counterstaining or in a non-counterstained section. Apart from this some dendritic spines of impregnated neurons or even some fine dendritic branches may be become visible by applying cresyl-violet.
All the Golgi stained sections can be stored at room temperature for only 1 year in the dark without even decreasing the staining intensity. Otherwise it’s a suggestion that these sections should be visualised immediately after the staining process in order to avoid any false results.
