Fetal Bovine Serum is used for Cell Cultured by laboratories as a culture Medium additive
that helps creating the right environment for cells to grow. Moreover, the fetal bovine
serum is the most widely used serum that has high quality of carrier proteins, hormones and
different other macromolecular proteins that helps in in-vitro cell culture of normal human
and mammal cells as well as cell lines. More specifically these serum additive helps in the
rapid growth of embryonic cells that are being transferred to the DMEM culture medium.
Apart from this these fetal bovine serums are being used from many years in order to clone
various animal cells. Fetal calf serum is also being widely use in different cell culture
applications due to its large variety of growth factors, cytokines and nutriments and also due
to low level of antibodies present in it that makes it more effective and efficient. Inactivated
bovine serum albumin is used in certain research applications and tests that need
inactivation of antibodies and treated by heat inactivation in which the serum is particularly
exposed to 56’C temperature for 30 minutes under different controlled conditions. Also
Serum albumin of other species like Mouse, rabbit, hamster, horse can be used.
The serum of these species contains all those proteins that are not involved in blood clotting
factors because it doesn’t contain any sort of blood cells either white or red. Also, these
serums have high level of electrolytes , antigens , hormones and different exogenous
substances that includes microorganisms and drugs that helps in the growth of the cells in
in-vitro conditions favourably.
Furthermore, Malondialdehyde modified bovine serum albumin is also a principle and the
most useful product in different research activities because of its functionality. These
products helps in a way that when it reacts with the cellular macromolecules like proteins,
DNA and RNA it reacts with DNA to form certain adducts in the form of deoxy guanosine and
deoxyadenosine that can be harmful and mutagenic so this property allows it to be used as a
marker of lipid peroxidation in different experiments that increases its usability in the field
of biotechnology to a greater extent.
It is used to grow cells better due to the presence of valuable cytokines, growth factors and
serum proteins that are effective in the growth of various eukaryotic cells in laboratories.
What should be the pH of the medium containing bovine serum albumin?
The pH of the medium containing bovine serum albumin should be between 6.5-7.5 that is an optimum pH for such type of medium.
For short and long term storage of malondialdehyde modified bovine serum what
Malondialdehyde modified bovine serum can be stored for longer and shorter time periods at a specific temperature of 4ºC.