It is a modification of McCoy′s 5A medium (or RPMI 1630) for cell culture
It is the abbreviation of the Roswell Park Memorial Institute where it was developed in 1966
It is used for Lymphocyte growth but also for adherent cells lines and normal cells.
Both normal or cell lines of lymphoblasts or adherent cells are cultured with RPMI
The pH is basic at 8 containing phenol red
Both normal and cell lines of all Eukaryote cells can be cultured with and CO2 incubator and RPMI
Vitamins, amino acids, glucose and salt. Gentaur supplies also RPMI without Tyrosine or Glutamine or Methionine.
RPMI has more Glucose and less Calcium, EMEM is the most diluted Basal Medium Eagle with even less glucose, vitamins and amino acids than DMEM.
No only Vitamins, amino acids and salts with Phenol red as pH indicator.
+4*C
HEPES is a goods buffer with pKa and keeps 5 % CO2 at pH8.
For most cell cultures the FBS is used as growth factor source.
