Mouse Genomic DNA is an universal external standard qPCR.
Yes the DNA will be degrade by the freeze thaw process.
cDNA is made from mRNA and displays no exons. cDNA studies the Transcriptome while Genomic DNA the Genome inclusive exons.
Most form mouse tails but also from mouse ears.
Is mouse genomic DNA suitable for genomic analysis, genomic library construction, and southern blotting? Whole genomy hybridication expetiments are performed with mouse genomic DNA.
A single DNA base pair (bp) is 0.65 kDA. This means that 1 mol of mouse DNA is over half a kilo, 650 grams! That’s big.
The excess of DNA will dilute too much the enzymatic reaction.
Is mouse genomic DNA suitable for PCR
It is the international gold standard for PCR
Gentaur genomic mouse DNA 260/280 ratios is less than 1.8, while the 260/230 ratios are higher than 2.0. RNA often is 60-80% of the DNA in homemade genomic mouse DNA samples, so you drastically overestimate your DNA content using fluorometry. Better use Gentaur genomic mouse DNA as international standard.
Gentaur uses Tris-EDTA and 10 mM Tris-HCl, pH8.5 at 25ºC for extraction.