Gentaur sells ready to use lentiviral particles for direct endocytosis. The RNA is released and transcribed into cDNA that in the nucleus will only integrate into dividing cells. We also sell the plasmids and with HEK293T cell to make your own and titrate the quantity.
For dividing cells its an easy way to integrate DNA
Lentiviral particles need a lipidic solution like transfectin or purefection or purefectin to integrate into the cellular membrane of eurcatiot cells
Both HIV- or FIV-based lentivector expression constructs are known
Germline transmission or Endogenous RetroVirus (ERV), is the integration into the genome of the host. This is used to bread knock-in or knock -out mouse depending of the place and DNA integrated.
Gentaur Produces the lentiviral particles in a BL2 for security reasons. These restrictions will be raised in 2022 due to our proprietary 4th generation packaging.
Second generation Lentiviral particles are made from one psPAX plasmid that expesses all HIV gag, pol, rev, and tat genes. For the 3th generation lenti particles construction different plasmids are used in Hek293Ts.
Yes transduction uses a lipidic transfection and no virus. Just use a lot of plasmid and by over presence it will express.
There are cased of wild endogenous lentiviruses but this is very rarely reported. Till than for second generation plasmids a BL2 is preferred.
The best way to study long-term protein expression is to order your stable cell line from Gentaur that expresses your gene of interest.
This is called germline transmission.
Lenti-Easy Expression System allows production of replication-incompetent
3rd generation lentivirus that can stably transduce both dividing and
non-dividing mammalian cells with high efficiency.
Only dividing cells are easy subject to infection.
All Packaging plasmids need to be present, encode the virion proteins, such as the capsid and the reverse transcriptase. Another plasmid contains the genetic material to be delivered by the vector.
No, you need to order separately the plasmid vector and particle from Gentaur. The particle being easier and cheaper because the plasmid can be multiplied infinitely.
transduction units (TU) per milliliter. 1TU = 1 infectious particle, so if 106 TU are used to infect 106 cells, then the MOI = 1. If 5 x 106 TU are used to infect the same number of cells, then the MOI = 5.
Particles can be immediately transducted, they are correctly titrated, you do not need a BL2 environment they can be used in any lab.
E. coli MDS42 clearly outperforms DH5α and TOP10 and is equivalent or better than STBL3 at both 29° and 36°
